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Outline why protein fractionation is a key step in sample preparation for proteomic analysis and describe...

Outline why protein fractionation is a key step in sample preparation for proteomic analysis and describe fractionation methods that are suitable for (a) biofluids and (b) cells/tissue.

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Part a. Protein fractionation is important in proteomic analysis because it removes the abundant component of proteome and later on it does the fractionation of proteins which are abundant or low in concentration.

Part b fractionation method for biofluids includes automated SPE-RP-HPLC technique which is an NMR based metabonomic approach that helps in the measurements of hundreds of metabolites in the bio fluid. It's is a very efficient and effective technique used these days.

Part c. There are numerous kits available for the cell fractionation of cells or tissues. These kits includes Subcellular Protein Fractionation Kit which helps us to enable a stepwise extraction and separation of the ytoplasmic membrane, chromatin bound, nuclear-soluble,and cytoskeletal proteins from the cultured mammalian cells or tissues

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