Question

You have isolated a nonsense suppressor that maps to a the tRNA-leu gene. If the suppressor has a single point mutation in it, and it suppresses the 5’ UAG stop codon then,

(a) what is the sequence of the anticodon in the mutated tRNA-leu gene and what was the original sequence before mutation? Show your work (codon-anticodon base pairing for example).

(b) Would this suppressor also suppress termination of the UAA stop codon? Explain.

Answer 1

Please look in the image for solution: