Question

use Flavourzyme to hydrolysis protien，the flavourzyme is powder, the flavourzyme need to make stock and add into substrate solution， or can directly add into the substrate？？？

Answer 1

Flavourzyme:

Flavourzyme is commercial proteases preparations produced from Aspergillusoryzaestrains, produced by fermentation of deep surface without genetic mutations and are used for hydrolysis of protein under the condition of neutral or slightly acid.

Flavourzyme is an aminopeptidase, a mixture of peptidase, medium-sized,endopeptidase and exopeptidase.It is possible to over 70% of hydrolysis of peptidelinkages. Flavourzyme hydrolyzed products created from being mixed with bitterpeptides are diverse, mostly small peptides and amino acids.

For the survey of the process of hydrolysis of protein from soybeans we use cellulast 1, 5L in order to break down the cell wall facilitates proteaseexposure and hydrolysis of organic substance.we need to make a stock out and add the substrate into to it and then they can be analysed.