Question

Why in an immunostaining procedure for a specific antigen do you normally use two antibodies sequentially? Please answer in specific details typed out! Thank you very much!

Answer 1

Answer:

Why in an immunostaining procedure for a specific antigen do you normally use two antibodies sequentially?  

• Immunostaining procedure usually requires two antibodies -- Primary and Secondary antibody.
• The primary antibody is unlabeled wheres the secondary antibody is labeled.
• The sequential use of primary and secondary antibodies increases the sensitivity of the immunostaining procedure because each primary antibody consists of several epitopes. As a result, many secondary antibodies can bind simultaneously to the primary antibody.  
• This sequential use of antibodies enables signal amplification and easy detection of antigen.