Question

PALA prevents aspartate from binding from binding to the enzyme but aspartate alone does not block PALA binding. Describe the order of substrate binding to Atcase.

Answer 1

-Atcase is Aspartate Transcarbamoylase Enzyme,which catalyzes the condensation of aspartate and carmoyl phosphate,which is the first step of synthesis of PYRIMIDINE

-In mammals Atcase forms part of the CAD which catayzes several steps in Pyrimidine biosynthesis,since pyrimidine is essential for DNA synthesis.

-Atcase inhibitors such as PALA has potential for slowing tumor growth in cancer

- Aspartate binds to active site,which leads to conformational change that close the active site and bring the substrate together for catalysis