Question

how should a plate for antimicrobial susceptibility testing be inoculated

Answer 1

Kirby-Bauer method is use din microbiology laboratory to test for antibiotic sensitivity. Small wafers containing antibiotics are placed onto a plate upon which bacteria are growing. If the bacteria are sensitive to the antibiotic, a clear ring, or zone of inhibition, is seen around the wafer indicating poor growth.

To inoculate the plate firstly prepare the inoculum from the primary culture plate by touching with a loop the tops of each of 3-5 colonies, of similar appearance, of the organism to be tested and transfer this growth to a tube of saline. If the inoculum has to be made from a pure culture, suspend a loopful of the confluent growth similarly.