Question

1. Use the numbers in the choices below to indicate where in the schematic diagram of a eukaryotic cell those processes take place. Write each number 1-5 next to “nucleus,” “ribosomes,” or “endoplasmic reticulum.”

transcription

translation

RNA splicing

polyadenylation

RNA capping

2. (True / False) A linear chromosome can have multiple origins of replication.

3. Part of the RNA sequence below encodes the N-terminal region of a protein, but not all of the nucleotides shown are translated into protein. Given this sequence, and your knowledge of the mechanism of translation, which amino acid will be attached to the first tRNA that enters into the A site during elongation?

5′-GUUUCCCGUAUACAUGCGUGCCGGGGGC-3′

a) methionine (b) arginine (c) cysteine (d) valine

Answer 1

1).

transcription--Nucleus

translation----Ribosomes

RNA splicing---Nucleus

polyadenylation---Nucleus

RNA capping ---Nucleus

2). True

Explanation:

Eukaryotes often have multiple origins of replication on each linear chromosome that initiate at different times (replication timing), with up to 100,000 present in a single human cell.

3). b. Arginine

Explanation:

Translation strats from AUG codon of mRNA from 5' end. AUG codes for methionine, which is attached at P site at first. The next codon in sequence is CGU, which codes for arginie and it occupies at A site during elongation.