1. Conduct a dihybrid cross of pea plants with the following combination of traits: Parent 1—Tall plant with white flowers (pure breeding); Parent 2—Dwarf plant with purple flowers (pure breeding). i) What are the genotypes of the parents? ii) What are the genotypes of their gametes? iii) Portray a cross and determine the F1 generation. Use a Punnett’s square. What are the genotypes and phenotypes of their offspring? iv) Conduct a F2 cross with the offspring. What are the genotypes and phenotypes of the offspring and their ratios.
Parent 1 (TTpp) x Parent 2 (ttPP)-----P1
Tp tP --------Gametes
TtPp (tall, purple)------F1
TtPp x TtPp (self cross)
TP |
Tp |
tP |
Tp |
|
TP |
TTPP (tall, purple) |
TTPp (tall, purple) |
TtPP (tall, purple) |
TtPp (tall, purple) |
Tp |
TTPp (tall, purple) |
TTpp (tall, white) |
TtPp (tall, purple) |
Ttpp (tall, white) |
tP |
TtPP (tall, purple) |
TtPp (tall, purple) |
ttPP (dwarf, purple) |
ttPp (dwarf, purple) |
tp |
TtPp (tall, purple) |
Ttpp (tall, white) |
ttPp (dwarf, purple) |
ttpp(dwarf, white) |
F2 phenotypic ratio:
Tall, purple : Tall, white : Dwarf, purple : Dwarf, white = 9:3:3:1
Genotypic ratio:
TTPP=1
TTPp = 2
TtPP = 2
TtPp = 4
TTpp = 1
Ttpp = 2
ttPP = 1
ttPp = 2
ttpp = 1
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