Question

Describe how the zone of inhibition is formed in the disc diffusion assay

Answer 1

The disk diffusion agar method is used to determine the effectiveness of antibiotics different microorganism which is done specifically. In this method, bacteria is made to grow on the agar media, and the area around every antibiotic plate is representative of the lethality of that antibiotic on the specific bacteria. Some of the factors affecting zone of inhibition are Inoculum density, timing of disc application, temperature of incubation, incubation time among others.