Column: A B C D E
F2 Phenotype |
# Observed |
Corresponding Genotype |
P or SCO or DCO |
Genotype Written in Correct Order on Homologs |
yellow |
310 |
ywv+l+ |
P |
|
wildtype |
32 |
|||
yellow, wavy |
150 |
|||
wavy, large |
320 |
|||
wavy |
16 |
|||
large |
120 |
|||
yellow, large |
14 |
|||
yellow, wavy, large |
38 |
|||
Total |
1000 |
a) How many sets of reciprocal phenotypes are shown? ____________________
b) In Column C, list the corresponding genotype. Account for all three traits. The first one has been provided for you as an example. For all answers in column C, use the order of traits: fur color, fur texture, ear size.
c) Indicate the type of each homolog in Column D: parental P, single crossover SCO, or double crossover DCO. The first one has been provided for you as an example.
d) On this form below or on blank paper to be submitted with this form, determine the correct order of all three genes. All work must be shown for credit. Fill in Column E above.
e) After you have determined the correct order of alleles on the parent homologs, use this to determine the map distance between the gene in the middle and those on either side of it. Set up and show all calculations. Draw the map and label the distances between the alleles:
F2 phenotype | # observed | Corresponding genotype | P or SCO or DCO | Genotype written in correct order |
yellow | 310 | ywv+l+ | P | ywl+v+ |
wildtype | 32 | yw+v+l+ |
SCO |
yw+l+v+ |
yellow, wavy | 150 | ywvl+ | SCO | ywl+v |
wavy, large | 320 | yw+vl | P | yw+lv |
wavy | 16 | yw+vl+ | DCO | yw+l+v |
large | 120 | yw+v+l | SCO | yw+lv+ |
yellow, large | 14 | ywv+l | DCO | ywlv+ |
yellow, wavy, large | 38 | ywvl | SCO | ywlv |
Total |
1000 |
Distance between yw and l
38+32+14+16/1000 * 100 = 10cM
Distance between l and v
150+120+14+16/1000 * 100 = 30cM
Gene order
yw------10cM-----l-------------------30cM-----------------v
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