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A buffer made of 250 mM sucrose and 50 mM sodium phosphate is used to homogenize...

A buffer made of 250 mM sucrose and 50 mM sodium phosphate is used to homogenize the tissues for this experiment. You have 2M sodium phosphate buffer and dry sucrose. How would you make 750 ml of the buffer? Show your calculations. (HINT: there is one piece of information that is NOT given that you need to find out in order to answer this)

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Answer #1

Please look in the image for solution:

Now, you have to take 18 ml of the solution from sodium phosphate buffer and add 250ml of water in it.

now add 64.1625 gram of sucrose sugar in it and dissolved it properly. Now, diluted it to 750 ml by adding water in it. Now dissolve properly.

in this way, 250mM sucrose and 50 mM sodium phosphate of 750 ml buffer solution is prepared.

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