Question

You would like to stain Cadherins and Integrins in the same cell for immunofluorescence analysis. Which...

You would like to stain Cadherins and Integrins in the same cell for immunofluorescence analysis. Which of the following antibody combinations would allow you to do this staining?

(1 point)

a)Combo #1: mouse-anti-rabbit, goat-anti-mouse-GREEN
Combo #2: mouse anti-integrin, goat-anti-mouse-RED

b)Combo #1: mouse-anti-Cadherin, goat-anti-mouse-RED
Combo #2: rabbit-anti-Integrin, goat-anti-mouse-GREEN

c)Combo #1: mouse-anti-Cadherin, goat anti-mouse-RED
Combo #2: goat anti-Integrin, goat anti-mouse-RED

d)Combo #1: mouse-anti-Cadherin, goat-anti-mouse RED
Combo #2: rabbit-anti-Integrin, goat anti-rabbit-GREEN

e)Combo #1: rabbit anti-Cadherin, goat anti-rabbit GREEN
Combo #2: rabbit anti-Integrin, goat anti-rabbit RED

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Answer #1

Ans,. d) always the primary antibody should be anti of my desired protein to be detected. And the secondary should be always anti of primary antibody. Then only the binding will occur. Because primary antibody binds the protein and secondary antibody binds primary. Among the options only d is correct. In combo 1 primary antibody is anti Cadherin from mouse and secondary is anti mouse antibody from goat. A red tag is there which will help to detect Cadherin. For detecting integrin, the combo 2 consists of anti integrin antibody from rabbit. Secondary is anti rabbit antibody from goat. The green tag will help to detect integrin. The two colours will help to seperate both the proteins

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