Question

A.) The presence of 32P in the pellet after centrifugation of the infected E. coli cell/virus...

A.) The presence of 32P in the pellet after centrifugation of the infected E. coli cell/virus mixture indicates…

A.Viral DNA is inside the bacteria

B.Viral protein coat is inside the bacteria

C.Viral DNA is outside the bacteria

D.Viral protein coat is outside the bacteria

B.) The absence of 35S in the pellet after centrifugation of the infected E. coli cell/virus mixture indicates…

A.Viral protein is inside the bacteria

B.Viral protein is outside the bacteria

C.Viral DNA is outside of the bacteria

D.Viral DNA is inside the bacteria

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Homework Answers

Answer #1

The above two questions are related with the Hershey and Chase experiment. DNA possess phosphorus but not protein and protein possess sulphur but not DNA.

Hershey and Chase made two types of viruses - one with radioactive protein coat (S35) and other with radiactive DNA (P32). Theses viruses were then allowed to infect fresh cultures of bacteria.The culture was then centrifuged during which bacterial cells being heavier got collected in the pellet and lighter viruses got collected in supernatant.

A) ans is A. Viral DNA is inside the bacteria. Since DNA is the genetic material it is passed from viruse to bacteria and thus found in pellet with bacteria.

B) ans is B. Viral protein is outside the bacteria. Since protein coat of virus is labelled with S35 and proteins are not the genetic material. Thus it is left in the supernatant with the virus.

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