Question

1) What effect would increasing the amount of agarose in the gel have on the distane...

1) What effect would increasing the amount of agarose in the gel have on the distane that pieces of DNA would migrate in the gel?

2) What was the most difficult problem you encountered when loading the samples into the wells?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

1. By increasing the amount of agarose in the gel, the distance travelled by the pieces of DNA will be reduced. Increasing the agarose concentration will lead to creation of smaller pores in the gel matrix. Due to this, the DNA will be able to migrate a shorter distance.

2. It is difficult to load the sample into the well because we are trying to place the sample into a clear well that is surrounded by a clear gel. Therefore the sample gets loaded into the gel instead of the well.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
You’ve been asked to prepare the gel. How much agarose would you need to weigh in...
You’ve been asked to prepare the gel. How much agarose would you need to weigh in order to prepare 50 ml of 2% gel? Show your math. List three things to which one needs to pay special attention when loading a gel for DNA electrophoresis. What would be the volume of the PCR sample you would have loaded in live lab? The loading buffer has a dual purpose. Explain. There are multiple reasons for loading a DNA ladder (size standard)...
When adding your DNA samples to the agarose gel, you will need to add loading dye...
When adding your DNA samples to the agarose gel, you will need to add loading dye to the samples. Loading dye contains: ¥ glycerol, which helps the samples sink into the sample wells of the gel; and ¥ tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis. The recipe for 6X loading dye is as follows: ¥ 0.25% bromophenol blue (w/v) ¥ 0.25% xylene cyanol (w/v) ¥ 30%...
We cannot observe the DNA with naked eye during run on agarose gel electrophoresis since DNA...
We cannot observe the DNA with naked eye during run on agarose gel electrophoresis since DNA is colorless and very low amount of DNA is used in this technique. a) Considering the charge of DNA, what is the direction of DNA migration when it is run on agarose gel electrophoresis? (5 pts) b) How do we observe the DNA on agarose gel electrophoresis if DNA is colorless? (5 pts) c) If you want to run an oligonucleotide (25 bp) on...
Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use...
Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use gel electrophoresis to compare the size of 2 dye molecules Methyl orange and Ponceau G. You will also analyze an “unknown” sample that contains a mixture of two dyes. Dye molecules with lower molecular weight or greater electrical charge will migrate faster through the gel, than dye molecules with greater molecular weights or lesser electrical charge. Materials: Mini gel electrophoresis chamber 6 Tooth comb...
What amount of agarose (g) and TAE buffer (mL) would you need to make a 1%...
What amount of agarose (g) and TAE buffer (mL) would you need to make a 1% gel?
In order to separate two DNA fragments of a similar size, what kind of agarose gel...
In order to separate two DNA fragments of a similar size, what kind of agarose gel would you use and would you run it for a short time or a long time?
1) A higher concentration of agarose (and hence smaller pore size in the gel) will allow...
1) A higher concentration of agarose (and hence smaller pore size in the gel) will allow you to separate what more efficiently? please list examples with explanation. 2) What could be a problem when trying to clone a 3.5kb fragment into a 3.5kb vector? Examples and explanations please. Thank you.
Electrophoresis Virtual Lab (11 Pts) 1. DNA will move toward which color electrode? What electrical charge...
Electrophoresis Virtual Lab (11 Pts) 1. DNA will move toward which color electrode? What electrical charge does this pole have? What about the structure of DNA, specifically, is responsible for this behavior? (3) 3. What is the purpose of a DNA standard in your gel? (2) 5. Let’s analyze the following scenarios: A. gels were prepared and the DNA samples, along with a DNA standard were placed in the wells. After an hour running the gel, the samples seem to...
1. What are the advantages of Y-chromosome STR profiling? Select the two correct answers. Less genetic...
1. What are the advantages of Y-chromosome STR profiling? Select the two correct answers. Less genetic variability exists on the Y chromosome than on autosomal chromosomes. Y chromosome STR profiling is possible for more than 200 loci. Gender separation of mixed tissue sample is easily achieved. Y chromosome STR profiling is more sensitive than standard STR profiling. 2. What are the limitations of this procedure? Y chromosome doesn't undergo recombination, thus it is difficult to differentiate DNA from close male...
1. what effect does increasing the sample size have on the population standard deviation? If the...
1. what effect does increasing the sample size have on the population standard deviation? If the sample size increases then the sample standard deviation will be decreases. But in this sentence, it is not a sample standard deviation. Every answer in CheggStudy is decrease. I think sample size doesn't affect to the population standard deviation. 2. When performing a hypothesis test, what effect does increasing the sample size have on the probability of type 1 error? 3. When performing a...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT