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Always sterilize your inoculating loop by flaming before using it to enter any culture material. not a fresh but used a sterile loop .Flame the loop after you streak each quadrant
In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified,
In this method the inoculated loop or swab is used to streak the sample many times in a zig-zag over the surface of a solid culture medium in a Petri dish.
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