Question

In defining the pink synthesis pathway, you find an intriguing large protein, Alecia. You scribble down the mRNA sequence in your lab notebook with a flair pen. Your lab partner bumps into you, and you accidently spill a bottle of liquid yeast media onto your notebook, blurring all but the middle of the mRNA sequence: 5’-AUGUGUAGGCACUGA-3’. What is the amino acid sequence of this region of the protein? Explain your answer.

Answer 1

5’-	AUG	UGU	AGG	CAC	UGA	-3’
NH2+	Met	Cys	Arg	His	stop	Coo-

Explanation: As we know that mRNA is synthesized in the 5’ to 3’ direction from the DNA and the protein synthesis also starts from 5’ to 3’ direction from mRNA making protein from N (NH₂⁺) terminal to C (Coo^-) terminal. In the mRNA sequence given in the question, the mRNA will be read in the 5’ to 3’ direction and making the sequence given in the table and last codon will be stop codon. (NH₂⁺5’- Met- Cys- Arg- His- Coo^— 3’