Question

Describe (in at least one sentence each) 3 ways that PCR is similar to DNA replication in cells

Answer 1

1) DNA replication: Replication of DNA Invivo, PCR is replication of DNA invitro and both require enzymes and primers for the process

2) 1st step involved in both is opening/melting the double strands of DNA Helix, in DNA replication it is done by helicase, in PCR it is by heating to nearly boiling temperatrue.

3) Both DNA Replication in cell nad PCR use DNA polymerase enzyme for the polymerization of individual nucleotide bases, to copy DNA.

4) Both DNA Replication and PCR works on the basis of base pairing rule(A pairs with T by two hydrogen bonds and G pairs with C by three hydrogen bonds).

5) Replication in cells and PCR, proceeds in 5'-3' direction and strand are antiparallel to each other.