Put the steps of initiation of DNA replication in their correct sequence.
- 1. 2. 3. 4. 5.
DNA polymerase catalyzes the synthesis of new DNA by adding deoxyribonucleotides, beginning by attaching the first to the 3' hydroxyl (-OH) of the last ribonucleotide in the primer.
- 1.
2. 3.
4. 5.
A specific set of many proteins recognizes the specific sequence that marks an origin of replication.
- 1.
2. 3.
4. 5.
The enzyme DNA helicase breaks the hydrogen bonds between the complementary base pairs and begins to separate the two anti-parallel DNA strands to form two replication forks.
- 1.
2. 3.
4. 5.
SIngle-strand binding proteins keep the complementary base pairs from forming hydrogen bonds, while topoisomerases repeatedly cut the DNA, allow it to unwind, and re-anneal it in front of the advancing replication fork.
- 1.
2. 3.
4. 5.
The enzyme primase synthesizes a short stretch of about 20 ribonucleotides, a primer, from 5' to 3' along each 3' to 5' DNA template.
steps of initiation of DNA replication :
Step1:
A specific set of many proteins recognizes the specific sequence that marks an origin of replication.
Step2:
The enzyme DNA helicase breaks the hydrogen bonds between the complementary base pairs and begins to separate the two anti-parallel DNA strands to form two replication forks.
Step3:
SIngle-strand binding proteins keep the complementary base pairs from forming hydrogen bonds, while topoisomerases repeatedly cut the DNA, allow it to unwind, and re-anneal it in front of the advancing replication fork.
Step 4:
The enzyme primase synthesizes a short stretch of about 20 ribonucleotides, a primer, from 5' to 3' along each 3' to 5' DNA template.
Step 5:
DNA polymerase catalyzes the synthesis of new DNA by adding deoxyribonucleotides, beginning by attaching the first to the 3' hydroxyl (-OH) of the last ribonucleotide in the primer.
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