Question

Put the steps of initiation of DNA replication in their correct sequence.       -   ...

Put the steps of initiation of DNA replication in their correct sequence.

      -       1.       2.       3.       4.       5.      

DNA polymerase catalyzes the synthesis of new DNA by adding deoxyribonucleotides, beginning by attaching the first to the 3' hydroxyl (-OH) of the last ribonucleotide in the primer.


      -       1.       2.       3.       4.       5.      

A specific set of many proteins recognizes the specific sequence that marks an origin of replication.


      -       1.       2.       3.       4.       5.      

The enzyme DNA helicase breaks the hydrogen bonds between the complementary base pairs and begins to separate the two anti-parallel DNA strands to form two replication forks.


      -       1.       2.       3.       4.       5.      

SIngle-strand binding proteins keep the complementary base pairs from forming hydrogen bonds, while topoisomerases repeatedly cut the DNA, allow it to unwind, and re-anneal it in front of the advancing replication fork.


      -       1.       2.       3.       4.       5.      

The enzyme primase synthesizes a short stretch of about 20 ribonucleotides, a primer, from 5' to 3' along each 3' to 5' DNA template.

Homework Answers

Answer #1

steps of initiation of DNA replication :

Step1:

A specific set of many proteins recognizes the specific sequence that marks an origin of replication.

Step2:

The enzyme DNA helicase breaks the hydrogen bonds between the complementary base pairs and begins to separate the two anti-parallel DNA strands to form two replication forks.

Step3:

SIngle-strand binding proteins keep the complementary base pairs from forming hydrogen bonds, while topoisomerases repeatedly cut the DNA, allow it to unwind, and re-anneal it in front of the advancing replication fork.

Step 4:

The enzyme primase synthesizes a short stretch of about 20 ribonucleotides, a primer, from 5' to 3' along each 3' to 5' DNA template.

Step 5:

DNA polymerase catalyzes the synthesis of new DNA by adding deoxyribonucleotides, beginning by attaching the first to the 3' hydroxyl (-OH) of the last ribonucleotide in the primer.

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