Fructose -2,6-bisphosphate activates the enzyme and high
ATP inhibits the enzyme.
Phosphofructokinase one of the main
regulatory enzyme of the glycolytic pathway. It is involved in the
conversion of fructose 6 phosphate to fructose 1, 6 bisphosphate.
This enzyme allosterically regulated by many activators and
inhibitors. The Phosphofructokinase is activated by the ADP, AMP,
cyclic AMP, NH3, high pH, fructose 6 phosphate, fructose 1,6
phosphate, fructose 2,6 phosphate. It is inhibited by the high ATP,
citrate, phopho enol pyruvate.