Question

Using the genetic code table, please answer the following questions:

a) Transcribe and translate the following DNA. Label any nucleic acids with their 5’ and 3’ ends.

DNA 3’ TACATAACCATT 5’

b) Using the DNA sequence above, write a new DNA sequence from 3’ to 5’ that incorporates a silent mutation.

c) Using the DNA sequence above, write a new DNA sequence from 3’ to 5’ that incorporates a nonsense mutation.

d) Using the DNA sequence above, write a new DNA sequence from 3’ to 5’ that incorporates a missense mutation in the second amino acid.

Answer 1

A- Upon transcription, we will get

5' AUGUAUUGGUAA 3'

Methionine- Tyrosine- Tryptophan- Stop codon

B- A silent mutation is a mutation in which upon mutation new codon translate same amino acid.

3' TAC ATG ACC ATT 5'

Methionine- Tyrosine- Tryptophan- Stop codon

C- A nonsense mutation leads to premature termination of translation

3' TAC ATT ACC ATT 5'

Methionine- Stop codon

D- Mis-sense mutation leads to translation of new amino acid.

3' TAC GTA ACC ATT 5'

Methionine- Histidine- Tryptophan- Stop codon