Question

What is the correct order of enzyme action during DNA replication? Number the steps from 1...

What is the correct order of enzyme action during DNA replication? Number the steps from 1 to 7.

_____ Synthesis of RNA primers (priming)
_____ Helicase/topoisomerase activity
_____ DNA polymerization/proofreading
_____ 5’-3’ exonuclease activity/DNA polymerization

_____ Ligation
_____ Single-strandedbindingproteinsbindand

prevent reannealing
_____ Binding to origin of replication (AT rich

sequence)

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Answer #1

first of all DNA become opens from the origin of replication region.

then helicase comes and opens the DNA and then single-stranded binding protein binds to the single strands of DNA.

after this RNA polymerase come and synthesise primer and then DNA polymerase continue to polymerization of DNA.

after the polymerization reaction proofreading activity was done to check the correct base pairing.

after all the reactions like ligase comes and ligate the strands of newly synthesized DNA.

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