Question

Evaluate the possibility of sterilizing milk at 110°C. Calculate the D-value for Clostridium botulinum at that temperature Then, Calculate the sterilization value at 121.1°C and how much time one should sterilize C. tuberculosis at 110°C.

Please show all steps of calculation

Answer 1

Ans: Milk is Sterilized for killing all microorganisms present, so that the packaged product can be stored for a long time at ambient temperature, without spoilage by microorganisms. Heating for 30 min at 110°C (in-bottle sterilization) inactivates all milk enzymes, but not all bacterial lipases and proteinases are fully inactivated; it causes browning, formation of a sterilized milk flavor, and some loss of available lysine; it reduces the content of some vitamins; causes considerable changes in the proteins including casein.

D-value for Clostridium botulinumat at temperature 110°C for 30 minute is D_110C =20 minutes.

Sterilization value at 121.1°C:

C. tuberculosis at 110°C should sterilize approximately 30 minutes.