It is difficult to isolate a particular type of bacteria from a clinical sample. Streak plate strategy is utilized to develop microorganisms on a growth media surface so individual bacterial colonies are isolated and diagnosed. Isolated provinces demonstrate a clone of cells, being gotten from a single antecedent cell. At the point when the chose culture media is inoculated utilizing a single isolated settlement, the subsequent culture develops from that chose single clone.
In 1st quadrant, there are so many species/strains of bacteria are present which can create confusion during diagnosis. So last isolated single colonies are selected.
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