Question

Using the general rules for bacterial promoter, identify the –10, -35 and first transcribed nucleotide (+1 A) on the sense strand of the E coli tRNA^Tyr
promoter shown below:
5’-CAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCATTTCCCGATA-3’
3’-GTTGCATTGTGAAATGTCGCCGCGCAGTAAACTATACTACGCGGGGTAAAGGGCTAT-5’

Answer 1

-35 Promoter region for sense strand of the E coli tRNA^Tyr are- TTTACA

AND -10 Promoter region for sense strand of the E coli tRNA^Tyr are- TATGAT

and spacer from initiation site to -10 region are-N₇

spacer between -10region to -35 region are -N₁₆

SO A/C to given sequences, these region are-

5’-CAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCATTTCCCGATA-3’

-35 region -10 region .   transcription start site.