Question

What will happen if you do not wear UV-blocking protective eyewear when viewing your gel on the UV-illuminator?

5. Why do you expect only a single band for each PCR reaction?

6. How are transgenes expressed in specific tissues within the embryo?

Answer 1

UV blocking protective eyewear protects against ultraviolet radiation,while providing optimum contrast in viewing and lessening eye fatigue .When you don't view your gel on UV illuminator by these eyewear there is a chance of risk at loosing your eyesight and some other radiations exposure to the skin .

5) We expect only a single band because sometimes the other band is due to nonspecific amplification which is against our experiments

6)The manipulated genes are developed in a special cells known as totipotent stem cells ,cells containing desired DNA are incorporated into the hosts embryo , resulting in a chimeric animal. Unlike the other methods,where we require live transgenic offspring but through this embryonic cell transfer can be tested