What will happen if you do not wear UV-blocking protective eyewear when viewing your gel on the UV-illuminator?
5. Why do you expect only a single band for each PCR reaction?
6. How are transgenes expressed in specific tissues within the embryo?
UV blocking protective eyewear protects against ultraviolet radiation,while providing optimum contrast in viewing and lessening eye fatigue .When you don't view your gel on UV illuminator by these eyewear there is a chance of risk at loosing your eyesight and some other radiations exposure to the skin .
5) We expect only a single band because sometimes the other band is due to nonspecific amplification which is against our experiments
6)The manipulated genes are developed in a special cells known as totipotent stem cells ,cells containing desired DNA are incorporated into the hosts embryo , resulting in a chimeric animal. Unlike the other methods,where we require live transgenic offspring but through this embryonic cell transfer can be tested
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