Question

Which of the following techniques can be used to isolate proteins? a. Salting out b. Size...

Which of the following techniques can be used to isolate proteins? a. Salting out b. Size exclusion chromatography c. Polyacrilamide gel electrophoresis d. All of these e. None of the above

Homework Answers

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
In a mixture of five proteins listed below, which should elute first in a size-exclusion chromatography...
In a mixture of five proteins listed below, which should elute first in a size-exclusion chromatography under non-denaturing conditions?   Note that the size-exclusion resin can only resolve proteins from 25 kDa to 200,000 kDa​ A. RNA polymerase, MW 450,000 Da B. Immunoglobin G, MW 145,000 Da C. Serum albumin, MW 67,000 Da D. Ribonuclease D, MW 17,000 Da E. Cytochrome c, MW 13,000 Da
you have following proteins protein PI MM Specific tag 1 6.4 28700 his6 tag 2 4.5...
you have following proteins protein PI MM Specific tag 1 6.4 28700 his6 tag 2 4.5 33100 no tag 3 6.5 210111 HIS6 TAG Which of the following methods would be the best method for separation? 2d gel electrophoresis, ion exchange size exclusion isoeletric focusing native page SDS page affinity chromatography 2D page
1. fill in the blank Researchers use a number of techniques to isolate or characterize proteins....
1. fill in the blank Researchers use a number of techniques to isolate or characterize proteins. For example,1. ____________________ separates molecules by size or charge, while 2.__________________ ____________________ exploits the attraction between protein and ligand. Primary level of protein structure can be determined by 3._______________________________, and secondary and tertiary structure can be delineated through 4. ____________________________________. 2. Match each molecule to its description. (1 point each) A. zymogen B. kinase C. cofactor D. substrate E. coenzyme 1. ___ organic molecule...
1. Which of the following answers is (are) true about size exclusion chromatography? a. large proteins...
1. Which of the following answers is (are) true about size exclusion chromatography? a. large proteins elute first b. small proteins interact more with the column c. proteins larger than the cutoff size do not elute d. will be used to purify GFP 2. Which of the following is (are) not true about ion exchange chromotagraphy? a. positively charged proteins elute first b. negatively charged proteins elute first c. uncharged proteins elute first d. hydrophobic proteins elute first 3. The...
1. Agarose gel electrophoresis can separate: A. DNA B. Protein C. DNA or proteins 2. Polyacrylamide...
1. Agarose gel electrophoresis can separate: A. DNA B. Protein C. DNA or proteins 2. Polyacrylamide gel electrophoresis can separate: A. DNA B. Protein C. DNA or proteins
Some characteristics of three proteins are listed in the table below: Protein Molecular Weight (Da) Isoelectric...
Some characteristics of three proteins are listed in the table below: Protein Molecular Weight (Da) Isoelectric point (pI) Does the Protein Contain a heme moiety? 1 65,000 5.5 No 2 54,000 10.0 No 3 14,500 4.5 Yes a.(2 points) What type of chromatography separates proteins based on size? b. (2 points) What type of chromatography separates proteins based on their charge? c.(10 points) Could gel filtration chromatography be used to separate a mixture containing Protein 2 and 3? Clearly explain...
34. Which of the following techniques uses capillary gel electrophoresis as an allele detection to distancing...
34. Which of the following techniques uses capillary gel electrophoresis as an allele detection to distancing between the reference allele and the variant one? A. Sanger DNA sequencing B. PCR-RFLP C. Pyrosequencing D. Tag Man
The chelator EDTA is often added to buffers that are used to isolate and purified proteins...
The chelator EDTA is often added to buffers that are used to isolate and purified proteins to inhibit proteases. Explain which family of proteases is going to be inhibited by EDTA and why. a) Asp proteases b) metalloproteases c) Ser proteases
1. Ethidium bromide has long been used for visualizing DNA. How does this molecule bind to...
1. Ethidium bromide has long been used for visualizing DNA. How does this molecule bind to DNA? a. Binds between histone molecules b. Binds to the nucleotide base c. Intercalates between the bases d. Binds to the phosphodiester backbone 2. What types of cells do we count using a hemocytometer? a. Yeast cells b. Human cells c. Bacterial cells d2 d. Virus particles e. A and B f. All of the above 3. Which of the following is most often...
6. Which of the following can be manipulated to promote amino acid production in microbes? A....
6. Which of the following can be manipulated to promote amino acid production in microbes? A. Alteration of promotor to increase binding of the repressor. B. Decrease in cell membrane permeability to prevent release of the amino acid. C. Alteration of enzymes in pathways such as the TCA cycle to halt the pathway and “push” the intermediate towards the production of the amino acid. D. A and C. 8. Which of the following is true about Polymerase Chain Reaction (PCR)?...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT