It’s demanding to measure the standard free-energy change associated with the hydrolysis of ATP because the minute amount of ATP remaining at equilibrium is difficult to measure accurately. The value of ΔG'º can be calculated indirectly, however, from the equilibrium constants of two other reactions (the first of which should look familiar) that have less favorable equilibrium constants:
glucose-6-phosphate + H2O à glucose + Pi Keq'=270
ATP + glucose à ADP + glucose-6-phosphate Keq'=890
Using this information, calculate ΔG'º for hydrolysis of ATP in units of kcal/mol.
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