Question

How do you determine a charge on ionizable amino acids: asp, arg, lys, his, glu. I...

How do you determine a charge on ionizable amino acids: asp, arg, lys, his, glu.

I know I have to compare P.H. vs Pk but I don’t know where to go from there.

Example: if the ph is 2 and glu pk= 4.3, would this make it negative since it’s acidic in nature?
—> P.H. 2 and His with pk of 6
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Answer #1

To determine charge on ionizable amino acid, please use the Henderson-Hasselbalch equation :

where is the dissociation degree. At pH=pKa, = 0.5 i.e. the rate at which H+ is dissociated. To calculate degree of dissociation, please use the equation:

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