Question

5. Draw a chain of two nucleotides, indicating the base used for each, and the location of the ester bonds between them.

a. Draw an incoming deoxycytosine triphosphate molecule, placing a box around the atoms that will be released during the chemical reaction of polymerization. b. Do you think this reaction is reaction is endergonic (energy absorbing) or exergonic (energy releasing)? Why?

6. S-CDK (cyclin A-CDK1) promotes the disassembly of the pre-RC by phosphorylating Cdc6 (targeting Cdc6 for degradation) and the origin. What other factor is released from the origin?

How does this help regulate DNA replication and prevent re-initiation?

Answer 1

Answer. 5 & a.

Answer. b

DNA replication is Exergonic, because the building blocks used in DNA sreplication are deoxyribonucleoside triphosphates (dNTP's).

Answer 6. Four factors: the origin recognition complex (ORC), Cdc6, Cdt1, and Mcm2-7 are releaed from the origin once the DNA replication has begin. They are synthesised only once per division in rsponse to cyclin-A.