Question

How would you prepare 50mL of a 3% agarose with 1XTBE WHEN YOU ONLY HAVE A BOTTLE OF 10XTBE IN YOUR LAB? (Hint: You need to first dilute with dH2O your the 10XTBE to 1XTBE)

Answer 1

(ANSWER)

(EXPLANATION)

AIM - Prepare 50mL of a 3% agarose with 1XTBE.

First prepare 1X TBE.

STOCK CONCENTRATION is 10X TBE

FINAL CONCENTRATION is 1X TBE

FINAL VOLUME is 50ml

C₁V₁ = C₂ V₂

C₁ = CONCENTRATION OF SOLUTION 1

V₁ = VOLUME OF SOLUTION 1

C₂= CONCENTRATION OF SOLUTION 2

V2 = VOLUME OF SOLUTION 2

10 x a = 1 x 50 ml

a = 50/10 = 5ml

5ml of 10X TBE is required to prepare 50ml of 1X TBE.

Preparation of 50ml of a 3% agarose in 1X TBE

3 % AGAROSE means 3grams of agarose is required for 100ml 1X TBE solution.

For 50ml of 1X TBE (3/100)*50 = 1.5 g

1.5g of agarose is required to prepare 3% agarose in 1X TBE.