How would you prepare 50mL of a 3% agarose with 1XTBE WHEN YOU ONLY HAVE A BOTTLE OF 10XTBE IN YOUR LAB? (Hint: You need to first dilute with dH2O your the 10XTBE to 1XTBE)
AIM - Prepare 50mL of a 3% agarose with 1XTBE.
First prepare 1X TBE.
STOCK CONCENTRATION is 10X TBE
FINAL CONCENTRATION is 1X TBE
FINAL VOLUME is 50ml
C1V1 = C2 V2
C1 = CONCENTRATION OF SOLUTION 1
V1 = VOLUME OF SOLUTION 1
C2= CONCENTRATION OF SOLUTION 2
V2 = VOLUME OF SOLUTION 2
10 x a = 1 x 50 ml
a = 50/10 = 5ml
5ml of 10X TBE is required to prepare 50ml of 1X TBE.
Preparation of 50ml of a 3% agarose in 1X TBE
3 % AGAROSE means 3grams of agarose is required for 100ml 1X TBE solution.
For 50ml of 1X TBE (3/100)*50 = 1.5 g
1.5g of agarose is required to prepare 3% agarose in 1X TBE.
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