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GENES HSP104 and PFK2 What is the phenotype of a knockout yeast (missing this particular gene)?...

GENES HSP104 and PFK2

What is the phenotype of a knockout yeast (missing this particular gene)? Or, if the knockout is not viable, what is the phenotype of a heterozygous yeast?

           

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Answer #1

HSP104 codes for an adenosine-binding heat-shock protein. It is involved in protein-folding and giving the yeast tolerance to heat. A knockout of this gene would result into a phenotype with reduced thermotolerance and therefore decreased fitness.

PFK2 codes for the beta subunit of a phosphofructokinase involved in glycolysis, and is absolutely essential for anaerobic growth. It is also important for induction of glucose-induced genes. A knockout would result into a slow-growing phenotype, unable to complete glycolysis, with decreased survival rate in the stationary phase. Certain genes requiring glucose as an inducer will not be expressed.

Both of these knockouts are viable, as HSP104 and PFK2 are categorized under non-essential genes.

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