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Which of the following statements about “triple-stranded” DNA is FALSE? a) It requires a specific DNA...

Which of the following statements about “triple-stranded” DNA is FALSE?

a) It requires a specific DNA sequence and set of conditions to form.

b) It is theorized to occur in DNA regions important for gene regulation.

c) It requires Hoogsteen pairing of the bases

d) It creates a helical structure.

e) It can never occur physiologically.

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Answer #1

Answer: b) It is theorized to occur in DNA regions important for gene regulation

  • In triple stranded DNA, the three oligonucleotides wind around each other to form a triple helix (d is true)
  • The third strand binds to a B form DNA double helix by forming Hogsteen base pairing (c is true)
  • The third strand binds to a highly specific sequence (for recognition) to the major groove of double helical DNA by forming Hoogsteen type bonds (a is true)
  • This compound is designed rationally to regulate gene expression artificially and does not occur physiologically (e is true)
  • It does not occur in DNA regions, but it is artificially constructed for gene expression regulation (b is false)
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