Question

7. explain the tetrazolium chloride test; identify the stages of seed germination; how to stimulate cuttings to develop roots; the effect of iodine solution on starch.

Answer 1

Tetrazolium chloride is used to differentiate between metabolically active and inactive seeds.
It is a white compound which turns into the red color by the activity of dehydrogenases produced by living tissue.
If seeds are viable, they turn white Tetrazolium chloride to red.

Stages of seed germination:
1. Elongation of radicle
2. Elongation of hypocotyl
3. Expansion of cotyledons
4. Exposure of plumule
5. Formation of leaves

Auxin induces adventitious roots on stem cuttings.

Iodine turns starch solution to blue colour.