Question

An irreversible inhibitor makes a covalent attachment to an amino acid residue at the enzyme active site. The Lineweaver-Burk data from such an inhibitor would look similar to which of these reversible inhibitors:

Group of answer choices

uncompetitive

noncompetitive I

competitive I

Answer 1

Competitive inhibitor. Competitive inhibitor binds to the active site of the enzyme non covalently . Enzyme and inhibitor form EI complex that is similiar to enzyme substrate Es complex. Only competitive inhibitor can bind to enzyme active site irreversibly. Where as non competitive inhibitor binds to the enzyme at a site other than active site. Uncompetative inhibitor binds to the enzyme substrate complex.

The irreversible competitor makes covalent attachment to the enzyme active site will look similar to the reversible competitive inhibitor in LIne weaver plot . However in case of of competitive inhibitor , inhibition can be overcome by increasing substrate concentration.