Question

What can you infer from the information presented in this table?

Proteins Involved in DNA Synthesis

Process	Protein	Function
Opening the helix	Helicase SS binding proteins Topoisomerase	Breaks hydrogen bonds between base pairs Stabilizes single-stranded DNA Breaks and rejoins DNA strand to untwist the double helix
Synthesis of leading strand	Primase DNA polymerase III Sliding clamp	Catalyzes the synthesis of the RNA primer Adds bases to the growing DNA strand Holds DNA polymerase in place during strand elongation
Synthesis of the lagging strand	Primase DNA polymerase III Sliding clamp DNA polymerase I Ligase	Catalyzes the synthesis of RNA primers Adds bases to the growing end of the Okazaki fragment Holds DNA polymerase in place during strand elongation Removes the RNA primer and replaces it with DNA Joins Okazaki fragments into a continuous strand

Options Are:

a) DNA polymerase I and DNA polymerase III have different functions.

b)	DNA polymerase I and DNA polymerase III are the same enzyme found in different organisms.

c)	The sliding clamp molecule is a ribozyme.

d)	Topoisomerase is involved in proofreading activity.

Answer 1

Answer- a) DNA polymerase I and DNA polymerase III have different functions.

Reason- DNA polymerase1 has different function than the DNA polymerase 3 . The function of DNA polymerase 1 helps to hydrolyse the RNA primer and adds the complimentary nucleosides where as DNA polymerase III has following functions:

A) adds deoxyribonucleoside triphosphates complementary to the DNA template strand.

B) Catalyses the phosphodiester bonds

C) profreads the deoxyribonucleotide against the template strand during it's synthesis.