Question

Which of the following are ingredients for PCR of a cancer gene? Buffer (Mg++, etc.) Oligonucleotide...

Which of the following are ingredients for PCR of a cancer gene?

Buffer (Mg++, etc.)

Oligonucleotide primers

Template/target DNA

dNTP (dATP, dCTP, dGTP, dTTP)

Cyclic AMP

Thermostable DNA polymerase

Uracil (U)
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Answer #1

The correct options are Template/target DNA and dNTP (dATP, dCTP, dGTP, dTTP).

There are only a few essential ingredients for PCR of a cancer gene : Template DNA, DNA primers, Deoxynucleotide triphosphates (dNTPs) and Thermophilic polymerase with a buffer.

The dNTPs are nucleotides adenine (ATP), guanine (GTP), cytosine (CTP), and thymine (TTP), which are linked to three phosphates in the place of one. It catalyzes the polymerization reaction by the break down off the other two phosphates and connects in the new base to pair with the template strand of DNA. The template DNA can be either genomic or plasmid DNA, or even another PCR product.

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