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Identify each of the enzymes involved in DNA replication and whether they are used during synthesis...

Identify each of the enzymes involved in DNA replication and whether they are used during synthesis of the leading strand, lagging strand, or both. And briefly explain their function during DNA replication. Enzymes: DNA Polymerases I and III, Topoisomerase, Helicase, Ligase, SSBPs

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Answer #1

DNA polymerase 1 removes the RNA primers from the fragments and replaces them with nucleotides required for DNA formation in 5' to 3' direction.

DNA polymerase 3 is essential for replication of leading and lagging strands.

Topoisomerases: They are involved in the overwinding or underfunding of DNA. They set right the altered DNA topology occuring during DNA replication and transcription.

Helicase: DNA helicases separate the double stranded DNA into single strands by breaking the hydrogen bonds between nucleotide bases.

Ligase : Enzyme that catalyses joining of DNA strands by catalyzing the formation of phosphodiester bond.

SSBPs: single stranded binding proteins

They bind single stranded DNA and help in formation of central nucleoprotein complex substrate for DNA replication, recombination and repair.

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