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Question: One of the tools in vector-based molecular cloning is “blue-white colony screening”. Describe what this...

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One of the tools in vector-based molecular cloning is “blue-white colony screening”. Describe what this method is used for and the molecular processes behind? And describes the purpose of the method and the molecular basis for appearance of blue and white colonies, respectively?

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Answer #1

The importance of blue white screening is to idenifty the recombinant bacteria.

In this blue white screeining, the plasmid contains gus gene (it also contians MCS or PLS), it produces b galactosidase enzyme which acts on X gal and turns the colony in to blue. When this plasmid inserted with foreign gene in gus MCS site, it leads to inactivation (it is reffered as insertional inactivation) of the product of gus gene, which fails to metabloize the X gal results the white color colinies.  

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