Question

The bacterium Clostridium difficile can lead to acute diarrheal disease and chronic inflammation in the colon known as colitis. Although it can be isolated from feces, the colon harbors more microorganisms than any other site in the human body. In order to culture C. difficile the following media should be used. a. agar b. liquid broth c. selective media d. enrichment media e. 25°C incubation temperatures

The answer choice can only be one and I do not know which it is. Any help is much appreciated.

Answer 1

Since, the colon harbours different kind of microorganisms, which can interfere with the isolation of one particular microorganism, we need to use a media which will promote the growth of a single microbe, while inhibiting the growth of others. Selective media is used to culture Clostridium difficile. This media is known as cycloserine cefoxitine fructose agar. It contains antibiotics, such as cycloserine and cefoxitine, which inhibits the growth of other microorganisms, and distinct colonies of C.difficile are obtained.