Critically discuss how a range of sequential techniques can determine the full complement of proteins in a cell and how such methodologies can be used to determine what may cause disease.
Answer) Proteomics is the analysis of the entire protein complement of a cell, tissues or an organism under a specific, defined set of conditions. IT includes mass spectrometry, protein fractionation, bioinformatics .It relies on the three basic technology in MS is used for the data necessary to identify the individual proteins and bioinformatics analyze and assemble the data. It determine the peptide sequence and it's results .
Proteomics is mainly used for the drug discovery and development through the comparative assessment of normal and diseased state tissues, transcription and expression profiling .target based process start with the selection of a protein targets based on its presumed and validated role in the relevant disease . Biochemical or biophysical assays are used to monitor modulation of target activity after hit validation lead to the compound it is being selected.
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