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Some proteins expressed at high levels form inclusions that are relatively insoluble. What is the most...

Some proteins expressed at high levels form inclusions that are relatively insoluble. What is the most effective way to facilitate purification of these proteins?

A. Decrease the number of insertions in the vector.
B. Use a reporter gene to locate the inclusion.
C. Produce the protein as a fusion protein.
D. Switch to an expression host with a larger intracellular volume.

Please explain why.

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