Question

Set up Bovine Serum Albumin dilutions (prepared in 0.01% SDS) to provide a standard curve for future lab experiments. Each group will be given 1 mL ampoules of 2 mg/ml BSA. We will have a few extra ampoules in case of accidents. Make your dilutions in 1.5 mL eppendorf tubes.

Final BSA Concentration	Concentration of BSA Stock	Desired Volume	Volume of Stock (to be added)	Volume of 0.01% SDS (to be added)
5ug/100uL		100uL
10ug/100uL		100uL
20ug/100uL		100uL
30ug/100uL		100uL
40ug/100mL		100uL
50ug/100uL		100uL
60ug/100uL		100uL
70ug/100uL		100uL

  

Answer 1

final BSA conc.	Conc. of BSA stock	Desired volume	Volume of stock (to be added)	Volume of 0.01%SDS to be added
5ug/100ul	2000ug/2000ul	100ul	0.25ul	99.75ul
10ug/100ul	2000ug/2000ul	100ul	0.5ul	99.5ul
20ug/100ul	2000ug/2000ul	100ul	1ul	99ul
30ug/100ul	2000ug/2000ul	100ul	1.5ul	98.5ul
40ug/100ul	2000ug/2000ul	100ul	2ul	98ul
50ug/100ul	2000ug/2000ul	100ul	2.5ul	97.5ul
60ug/100ul	2000ug/2000ul	100ul	3ul	97ul
70ug/100ul	2000ug/2000ul	100ul	3.5ul	96.5ul