Question

Set up Bovine Serum Albumin dilutions (prepared in 0.01% SDS) to provide a standard curve for future lab experiments. Each group will be given 1 mL ampoules of 2 mg/ml BSA. We will have a few extra ampoules in case of accidents. Make your dilutions in 1.5 mL eppendorf tubes.

Final BSA Concentration |
Concentration of BSA Stock |
Desired Volume |
Volume of Stock (to be added) |
Volume of 0.01% SDS (to be added) |

5ug/100uL |
100uL |
|||

10ug/100uL |
100uL |
|||

20ug/100uL |
100uL |
|||

30ug/100uL |
100uL |
|||

40ug/100mL |
100uL |
|||

50ug/100uL |
100uL |
|||

60ug/100uL |
100uL |
|||

70ug/100uL |
100uL |

Answer #1

final BSA conc. |
Conc. of BSA stock | Desired volume | Volume of stock (to be added) | Volume of 0.01%SDS to be added |

5ug/100ul | 2000ug/2000ul | 100ul | 0.25ul | 99.75ul |

10ug/100ul | 2000ug/2000ul | 100ul | 0.5ul | 99.5ul |

20ug/100ul | 2000ug/2000ul | 100ul | 1ul | 99ul |

30ug/100ul | 2000ug/2000ul | 100ul | 1.5ul | 98.5ul |

40ug/100ul | 2000ug/2000ul | 100ul | 2ul | 98ul |

50ug/100ul | 2000ug/2000ul | 100ul | 2.5ul | 97.5ul |

60ug/100ul | 2000ug/2000ul | 100ul | 3ul | 97ul |

70ug/100ul | 2000ug/2000ul | 100ul | 3.5ul | 96.5ul |

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