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The five I's include Inoculation, Incubation, Isolation, Inspection, and Identification. Use a patient example to follow...

The five I's include Inoculation, Incubation, Isolation, Inspection, and Identification. Use a patient example to follow through the 5 I's?

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To identify the responsible pathogen to a given pathogenic condition in a patient,

1. Collect the biological sample which shows pathological symptoms (Assume that it has actively growing pathogen)

2. INOCULATE the sample into a culture medium.

3. INCUBATE the culture medium under optimal conditions for the pathogen to grow.

4. ISOLATE the pathogen from the culture medium.

5. INSPECTION: Observe the pathogen under the microscope and perform specific diagnostic tests.

6. IDENTIFY the pathogen based on the inspection results.

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