Question

Long Answer 1. You perform a Nirenberg and Ochoa type experiment in which synthetic RNAs are...

Long Answer

1. You perform a Nirenberg and Ochoa type experiment in which synthetic RNAs are made by incubating polynucleotide polymerase with CDP and UDP (80% CDP to 20% UDP ratio). What ratios of each of the eight possible codons do you expect?

2. What percentages of radioactivity would you expect for each of the 20 amino acids when a cell free translation assay is run 20 times in which each time a different radioactive amino acid is included with the 19 other non-radioactive amino acids?

3. You perform a Khorana type experiment with three different co-polymer RNAs made from 5’-UA-3’, 5’-AAU-3’, and 5’-UACA-3’polymers. After running 20 cell-free translations assays on these different copolymers what would you be able to conclude regarding codon usage? I don’t want you to tell me what the amino acids for each codon are but rather what Khorana would have concluded regarding codon usage. Hint: you will need to determine the codons present in each co-polymer and the expected amino acids that would be incorporated given the genetic code. Then, use the results to determine which amino acids could be definitively assigned to a codon and which could not be definitively assigned.   

Homework Answers

Answer #1

1) Using two types of nucleotides CDP, UDP The 8 posible codans are CCC, UUU, CUU, CUC, CCU,UCC, UCU and UUC.

2)cell free translation assay used for in vitro expression of proteins that means protein synthesis out of cells. If the adioctive amino acids are replaced for all amino acids in step wise manar than the whole protein product will be radioactive. Hence the radioactivity is hundred percent.

3) 5'UA3' Co polymer gives UAU AUA...repeating polymer which yields tyr-ile peptide polymer.

5' AAU3' polymer gives AAU AAU repeating RNA which yield poly peptide with asp repeating units.

5'UACA3' polymer gives 'UAC AUA CAU ACA repeating RNA which yield poly peptide with tyr- ile-his-thr amino acid repeating unit.

Together they utilise tyr,ile,asp,his,thr amino acids in reaction tube.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Translation: From RNA to Protein 1. How is it possible that 4 nucleotides can code for...
Translation: From RNA to Protein 1. How is it possible that 4 nucleotides can code for 20 amino acids? 2. What is meant by the fact that the genetic code is redundant? 3. How is tRNA involved in the process of transcription? 4. How is it possible that 31 different tRNAs can translate 64 different codons? 5. Describe the process by which tRNA and amino acids are joined. 6. What is the role of ribosomes in translation? Describe how the...
Consider a single murine hepatocyte at homeostasis with its surrounding environment, which includes other neighboring hepatocytes...
Consider a single murine hepatocyte at homeostasis with its surrounding environment, which includes other neighboring hepatocytes and extra-hepatic tissues. Due to changes in the organism’s activity, this hepatocyte receives signals that trigger the need to synthesize exactly 20 copies of a protein required for altered amino acid metabolism. PLEASE GIVE EXPLANATIONS FOR YOUR ANSWERS. THANK YOU! 1. How many equivalents of ATP are required to synthesize these 20 protein molecules? 2. Under aerobic conditions, how many molecules of glucose must...
12. If the DNA repair mechanisms fail to correct a defect in nucleotide sequencing, a permanent...
12. If the DNA repair mechanisms fail to correct a defect in nucleotide sequencing, a permanent change known as a __________________ may result. 13. As the enzyme helicase opens and “unzips” the two strands of DNA by breaking the hydrogen bonds, a Y-shaped ________________________ forms. a. Lagging strand b. Leading strand c. Okazaki fragment d. Replication fork 14. One large difference between transcription and translation between prokaryotes and eukaryotes is that, in prokaryotes: a. Translation occurs simultaneously with transcription b....
When is a hypothesis considered scientific? a. when it is based on something other than observation...
When is a hypothesis considered scientific? a. when it is based on something other than observation b. when it can be tested and is refutable c. when it relies on anecdotal evidence d. when it relies on mystical explanations e. All hypotheses are considered scientific until experiments determine otherwise. 3. Of the following, which is the earliest step in the scientific process? a. generating a hypothesis b. analyzing data c. conducting an experiment d. drawing a conclusion e. developing a...
9. Depression, hot flashes and cessation of the menstrual cycle are typical symptoms of _______.            a....
9. Depression, hot flashes and cessation of the menstrual cycle are typical symptoms of _______.            a. PMS b. Menopause c. Infertility d. ovarian cancer              10. A standard screening test for cervical cancer is called _______.                    Pap Smear       Genome sequencing ELISA NAAT testing              11. The actual gene sequence governing the expression of a trait is called a _______. a. Phenotype b. Genotype    c. Haplotype d. Molecular signature 12. The physical expression of this trait is the _______, which...
1. In which phase of the business cycle is the U.S. economy currently in? ________________. How...
1. In which phase of the business cycle is the U.S. economy currently in? ________________. How many months has the U.S. economy been in this stage of the business cycle? ___________ months 2. How long has the current expansion/recovery lasted to date? _________________ How does this compare to the average length of U.S. recessions since 1854? ______________________________. 3. What do the last four recoveries/expansions (that is, the current recovery/expansion and the previous three recovery/expansions), suggest about a new trend in...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT
Active Questions
  • ***Environmental Engineering*** Well water in Sullivan County, Pennsylvania was sampled and found to contain 6,590µg/L of...
    asked 3 minutes ago
  • A 25 mm diameter egg roll (k = 1 W/m degree Celsius) is roasted with the...
    asked 11 minutes ago
  • Instruments in airplane A indicate that, with respect to the air, the plane is headed 30°...
    asked 20 minutes ago
  • You wish to test the following claim (H1H1) at a significance level of α=0.05α=0.05.       Ho:μ=73.9Ho:μ=73.9       H1:μ≠73.9H1:μ≠73.9...
    asked 20 minutes ago
  • Let X1 and X2 be independent Poisson random variables with respective parameters λ1 and λ2. Find...
    asked 25 minutes ago
  • You wish to test the following claim (H1H1) at a significance level of α=0.001α=0.001.       Ho:μ=88.8Ho:μ=88.8       H1:μ>88.8H1:μ>88.8...
    asked 31 minutes ago
  • You have been asked to install floor tiles and paint your aunt’s bathroom based on the...
    asked 34 minutes ago
  • A new disinfection system is being designed for a treatment plant with a flow of 400...
    asked 34 minutes ago
  • A produce distributor uses 786 packing crates a month, which it purchases at a cost of...
    asked 42 minutes ago
  • What do researchers believe explains why some individual to engage in precocious, promiscuous, unprotected sex or...
    asked 42 minutes ago
  • 4. For a 99% confidence level, how large of a sample size is needed for a...
    asked 42 minutes ago
  • Another student was upset for a different reason. He thought that Test 2 was too long...
    asked 43 minutes ago