Question

Which sequence has a 2 prime OH group on the sugar?

CAGUACGUAGCUGA or TACCATCTACGTATA

Answer 1

The first sequence i.e CAGUACGUAGCUGA has a 2-hydroxyl group as this group is only present on RNA, not DNA( other sequence)

The additional 2’ hydroxyl (OH) group present in RNA, but lacking in DNA, has effects that range from the biogenesis of life on earth to how RNA oligonucleotides should be stored. Importantly, the addition of the 2’ OH group increases melting temperature (Tm) and stability by locking an RNA duplex into a compact A-form helix that is more stable than DNA’s standard B-form helix.