Question

If an enzyme reaction rate is approaching the Vmax, with a large excess of substrate present,...

If an enzyme reaction rate is approaching the Vmax, with a large excess of substrate present, which of the following would have the least effect on the rate of reaction?

Add moderate amount of a competitive inhibitor
Add a moderate amount of a non-competitive inhibitor
Add more enzyme to the mixture

Add a moderate amount of an irreversible inhibitor

Decrease the Temperature of the Reaction by a moderate amount

How can Vmax be approached despite the presence of a competitive inhibitor?

Increase enzyme concentration to very high levels
Increase substrate concentration to very high levels
Increase the temperature of the reaction
Decrease the temperature of the reaction
Add a non-competitive inhibitor to the reaction mixture
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a. If an enzyme reaction rate is approaching the Vmax, with a large excess of substrate present, which of the following would have the least effect on the rate of reaction?

(i)

add moderate amount of competitive inhibitor.

Competitive inhibitor work by competing substrate for active site. If substesub concentration is very high, then moderate addition of competitive inhibitor will have the least effect.
b.

How can Vmax be approached despite the presence of a competitive inhibitor?

(ii)

Increase substrate concentration to very high levels

High levels of substrate would outcompete competitive inhibitor.
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