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Regarding phosphofructokinase: a. How does ATP affect PFK1? b. How does ATP affect PFK 2? c....

Regarding phosphofructokinase:

a. How does ATP affect PFK1?

b. How does ATP affect PFK 2?

c. Why is serine 32 important to PFK2?

d. When would serine 32 be phosphorylated, during glycolysis or gluconeogenesis?

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Answer #1

A) PFK1 is the enzyme in glycolysis that conversation fructose 6 phosphate to fructose 1,6 bisphosohate . Atp is which is a result of glycolysis would this inhibit this commitment step of glycolysis.

B) PFK2 is the enzyme converts F6P to F-2,6-BP. This enzyme is not regulated by ATP

c) serine 32 determines the activity of bifunctional enzyme PFK2. In unphosphorylated state the enzyme favours glycolysis and in phophorylated state favours gluconeogenesis.

D) Fructose 2,6bisphosphatase activity of PFK2 decreases the levels of FBP which inhibits pFK1 inhibiting glycolysis and stimulating gluconeogenesis.

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