Question

Based on your knowledge of the chemical structure of DNA, explain why all DNA molecules move...

Based on your knowledge of the chemical structure of DNA, explain why all DNA molecules move in the same direction towards the positive electrode when placed in an electric field. Name one analysis tool in biotechnology which uses this feature.

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Answer #1

  Answer -

- The DNA molecules are negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.

* Analysis tools of Botechnological Methods -

An Gel electrophoresis is a technique used to separate DNA fragments according to their size. ... DNA fragments are negatively charged, so they move towards the positive electrode. Because all DNA fragments have the same amount of charge per mass, small fragments move through the gel faster than large ones.

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