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When doing genetic engineering to take a gene from a human cell and put it into...

When doing genetic engineering to take a gene from a human cell and put it into a bacteria, what would the new plasmid need to contain to be functional in the bacterial population and make the protein? List as many as are necessary?

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Answer #1

The plasmid should have a origin of replication,

Multiple cloning site or polylinker-This region contain many unique restriction sites and allow a gene to be conveniently inserted into the vector or removed from it.

A selectable marker -It allow the selection of positively transformed cells.

a reporter gene-It allow to identify successful clone

gene of interest in MCS region.

element of expression-It should contain promoter, ribosomal binding site (RBS),start codon,stop codon.

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