Question

A particular S. cerevisiae mutant strain cannot make the amino acid, histidine. Will the strain grow...

A particular S. cerevisiae mutant strain cannot make the amino acid, histidine. Will the strain grow on a YPD plate? what about a SD plate? Could you modify the SD plate to allow this strain to grow?

Homework Answers

Answer #1

YPD plate - contains all amino acids.

SD plate - minimal media: only the essential nutrients (i.e., no amino acids).

since the YPD contains all the amino acids so the mutant strain will grow on this plate also the nonmutated will grow on this plate.

SD contains all no amino acids so the mutant strain will not be able to grow on this plate but the nonmutated will grow on this plate. to allowing the growth of mutated one on SD plate we have to incorporate amino acid Histidine in the SD media plate.

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